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Evaluation of substances affecting turkey spermatozoa motility
THE following research by Peter Massanyi, To- mas Slanina and Michal Miskeje on the evalu- ation of substances af- fecting turkey sper- matozoa motility was submitted for the recent 2022 Australian Poultry Science Symposium.
colytic substrates and in- termediates of the citric acid cycle is not suffi- cient to ensure prolonged in vitro survival of turkey spermatozoa.
fects on the spermatozoa motility of domestic tur- keys – meleagris gallopa- vo domesticus – during in vitro incubation.
ning of incubation.
The concentration of
Turkey spermatozoa storage is successfully utilised in commercial breeding operations, but the efficiency of such systems in maintaining spermatozoa fertilising ability in vitro for 24 hours leads into insig- nificance in comparison to the oviductal storage system in vivo, which can maintain fertilising abil- ity for many weeks.
The majority of dilu- ents used are salt solu- tions convenient for im- mediate survival of sper- matozoa because they provide osmotic pressure and pH identical with the seminal plasma.
Significantly, a stimula- tory effect of taurine on motility parameters was observed in the samples containing 7.5, 5 and 2.5mg/ml of taurine.
Most of semen diluents provide the energy for metabolism and buffer- ing capacity, prevent clumping by thinning out the concentration as well as increasing the meta- bolic activity of the sper- matozoa and enhancing their motility.
Additionally, the age of turkey males affects the quality of fresh and also incubated spermatozoa.
The highest monitored concentrations of treha- lose 10mg/ml and fruc- tose 20mg/ml showed a relatively balanced value of motility parameters.
After 24 hours of in- cubation, significantly higher values of motility parameters were meas- ured in the extender three compared to the control.
From a practical stand- point, this reduces the normal number of males required and the overall costs.
The ageing process was accompanied by a reduc- tion of the number of spermatozoa and semen volume – decreasing mo- tility, viability and mem- brane integrity of sper- matozoa.
In the lower concentra- tion of trehalose 5, 2.5, 1.25mg/ml and fructose 5, 10, 15mg/ml, a nega- tive effect on the individ- ual motility parameters of turkey spermatozoa was observed.
In conclusion, we can state that we have suc- cessfully developed new turkey semen extenders, which not only maintain spermatozoa motility during short-term storage in in vitro condition, but also have a stimulatory effect on turkey sperma- tozoa motility.
In order to maintain the fertilisation ability of stored spermatozoa in vitro, semen has to be stored at 2-8C and dilut- ed in a suitable diluent.
Consequently, these changes lead to a pro- gressive decrease in the fertilising ability of tur- key semen and can also influence the preserva- tion during storage.
A significantly higher and progressive motil- ity of spermatozoa was detected in the samples containing caffeine rang- ing from 0.16mg/ml to 7.5mg/ml in comparison to the control sample at 5C.
For further information on this research, contact Peter Massanyi of the Slovak University of Ag- riculture in Nitra, Slovak Republic on peter.mas sanyi@uniag.sk
The use of low tem- peratures in combination with a buffered saline medium containing gly-
The aim of the study was to evaluate the im- pact of selected additive substances with the po- tential stimulatory ef-
At cultivation temper- ature 41C, the positive effect of caffeine was shown only at the begin-
Peter Massanyi, Tomas Slanina and Michal Miskeje
Diluents should also contain various energy substrates.
The primary aim of the study was to develop a new semen extender us- able in practice based on obtained results.
Following the continu- ous results, three new semen extenders were prepared.
Analysis of spermatozoa motility using a computer-assisted semen analyser system at the Institute of Applied Biology of the Slovak University of Agriculture in Nitra.
The image of detailed spermatozoa motility analysis using a specific turkey sper- matozoa setup.
Turkey spermatozoa motility in percentage in different semen extenders.
Therefore, diluents used for poultry semen are enriched with car- bohydrates – glucose or fructose – and other components probably to provide energy such as citrate, glutamate or ac- etate.
The individual motil- ity parameters were analysed by a computer- assisted semen analyser system at 5C and 41C in different time periods.
At the cool media in- cubation of 5C, all three extenders showed signifi- cantly higher values of all CASA motility pa- rameters from 1-5 hours during in vitro incuba- tion.
Specifically, the effect of different concentra- tions of trehalose, fruc- tose, caffeine and taurine diluted in the physiologi- cal solution as the culture medium were analysed.
taurine above 10mg/ml had a negative effect on spermatozoa motility.
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National Poultry Newspaper, April 2022 – Page 7